IL-2isaTcellstimulatorycytokinebestknownforinducingactivatedTcellproliferationinvitro.IL-2alsostimulatesproliferationanddifferentiationofBcells,naturalkillercells,monocytesandmacrophages.IL-2iscriticalforthedevelopment,survival,andfunctionofCD4+CD25+regulatoryTcells,whichpromotesTcelltolerancebysuppressingTcellresponsestoself-antigensinvivo,thusinhibitingimmuneresponsesandpreventingautoimmunediseases.IL-2playsanessentialroleinsensitizingTcellstoactivation-inducedcelldeath,aprocessmediatedprimarilybyFasandTNFsignalstolimitthemagnitudeofTcellexpansionthroughprogrammeddeathofactivatedTcells.IL-2bindstoandsignalsthroughmembranereceptorIL-2R,whichisexpressedalmostexclusivelybyactivatedT-cells.IL-2RisatrimericreceptorcomplexconsistingofthreedistinctsubunitsdesignatedasIL-2Rα(CD25),IL-2Rβ(CD122),andcommonγ-chain(γc,CD132).IL-2RαbindsexclusivelytoIL-2withlowaffinityandincreasesbindingaffinityofthewholereceptorcomplex,includingIL-2Rβandγcsubunits.IL-2RβisalsousedbyIL-15.Thecommonγcisusedbyothercytokines,includingIL-4,IL-7,IL-9,IL-15,andIL-21.BindingofIL-2initiatessignalingcascadesinvolvingJak1,Jak3,Stat5andthePI3K/Aktpathways(2-5).RecombinantHumanIL-2ACFhasaserinesubstituteforcysteineatposition126.RecombinantHumanIL-2ACFisanon-glycosylatedprotein,containing134aminoacids,withamolecularweightof15.5kDa.
- AccessionNumber:P60568
- Synonyms:Tcellgrowthfactor,TCGF,Aldesleukin
- Purity:≥90%determinedbyreducingandnon-reducingSDS-PAGEanalysis
- Bioactivity:TheED50istypicallylessthan1ng/mL.ED50isdeterminedbythedose-dependentproliferationofCD3+Tcells.
- Animalcomponent-freeandcarrier-free
- Custompackagingavailable
Forresearchorfurthermanufacturinguseonly.
Alsoavailableinothersizes.
