FLT-3Ligandisamemberofthe“potentiating”groupofhematopoieticgrowthfactorsthatstimulatetheproliferationofhematopoieticcells.Othermembersofthisfamilyincludestemcellfactor(SCF)andcolonystimulatingfactor1(CSF-1).FLT-3Ligandhasminimalproliferativeactivityonitsown,butcansynergizewithawiderangeofothercolonystimulatingfactorsandinterleukinstostimulateproliferation.FLT-3Ligandbindsto,andtransmitssignalsthrough,thereceptortyrosinekinaseknownasFMS-liketyrosinekinase-3(FLT-3).FLT-3LigandexertsamarkedstimulatoryactiononprimitivehematopoieticProgenitorcellsandauniqueexpandingeffectonputativestemcells.Thedistalproliferative/differentiativeactionofFLT-3Ligandislargelyrestrictedtogranulomonocyticprogenitorsandmonocyticprecursors.FLT-3Liganddoesnotaffectthegrowthoferythroidcommittedprogenitors.ExpressionoftheFLT-3receptorisessentiallyrestrictedtoearlyhematopoieticprogenitorcellsinaquiescentstatusandprogenitorsundergoinggranulomonocyticdifferentiation,suggestingdifferentialexpressionoftheFLT-3receptorunderliestheselectivestimulatoryeffectofFLT-3Ligand.FLT-3Ligandalsoplaysanimportantroleinlymphopoiesis.FLT-3Ligandpromoteslong-termexpansionanddifferentiationofhumanpro-BcellsinthepresenceofIL-7orincombinationofIL-7andIL-3.AtleasttwocytokineshavebeenidentifiedtoblockFLT-3Ligand-inducedorpotentiatedhematopoieticactivitys:TNF-alphaandTGF-beta(2-4).HumanFLT-3LigandcanstimulatetheproliferationofcellsexpressingmurineFLT-3receptors.RecombinantHumanFLT-3LigandACFisanon-glycosylatedproteincontaining155aminoacids,withamolecularweightof17.6kDa.
- AccessionNumber:P49771
- Synonyms:FLT3L,Fms-relatedtyrosinekinase3ligand
- Purity:≥98%determinedbyHPLC,reducingandnon-reducingSDS-PAGE,UVspectroscopyat280nm.
- Bioactivity:TheED50istypicallylessthan5ng/mL.ED50isdeterminedbydose-dependentproliferationofOCI-AML5cells.
- Animalcomponent-freeandcarrier-free
- Custompackagingavailable
Forresearchorfurthermanufacturinguseonly.
