TPOisa68-85kDglycoproteinthatsignificantlyexpandsbonemarrowandsplenicmegakaryocytesandtheirCD34+precursors,resultinginincreasedplateletproduction.TPOsupportsearlymegakaryocyte-ProgenitorcolonyformationandinducesexpressionofmegakaryocytedifferentiationMarkers,polyploidization,andmaturationintoplatelets.Encodedbytheproto-oncogenec-Mpl,theTPOreceptorc-MpllacksintrinsickinasedomainsbutTPObindingcharacteristicallyinducestyrosinephosphorylationofmultipleintracellularproteins.TheJanusfamilyofnon-receptorproteintyrosinekinases(JAKs)isthekeymediatorofTPOreceptorsignaling.TheN-terminalfirst153aminoacidsofTPOcontainstheentirereceptor-bindingregion,whileitsC-terminaldomainhasmulti-N-linkedglycosylationsitesandfunctionstopromoteTPOsecretionandprolongitscirculatorysurvival.TPOaffectstheentirethrombopoieticprocessandhasstrongereffectsinthelaterstages.Otherthrombopoieticcytokinesincludestemcellfactor(SCF),IL-3,IL-6,andIL-11.ThereisgreattherapeuticpotentialforTPOforthetreatmentofmanyclinicalconditionsthatresultinthrombocytopenia.Human,mouseanddogTPOshow69-75%aminoacidhomology.RecombinantHumanTPOACFisanon-glycosylatedprotein,containingthe174aminoacidsofthereceptorbindingdomain,withamolecularweightof18.6kDa(2-5).
- AccessionNumber:P40225
- Synonyms:MegakaryocyteColonyStimulatingFactor,c-MPLligand,MGDF
- Purity:≥95%determinedbyreducingandnon-reducingSDS-PAGE.
- Bioactivity:TheED50istypicallylessthan2ng/mL.ED50isdeterminedbydose-dependentproliferationofMO7ecells.
- Animalcomponent-freeandcarrier-free
- Custompackagingavailable
Forresearchorfurthermanufacturinguseonly.
Alsoavailableinothersizes.
