FreezingMediumTYBcontainsTEST-yolkbuffer(TYB)torecovermoremotilespermpost-thawthanglycerol-onlymedia(Hallak2000,Nallella2004).TEST-yolkbufferisasemenextenderandcryobufferthatcanbepairedwithglyceroltoachievehighspermmotilityandviABIlity.TheeggyolkisfromUSDAcertifiedvirus-freeflocksandwashedfromthespermaftercryopreservation.FreezingMediumTYBisalsotheonlyCE-markedandFDA-approvedspermfreezingmediacontainingTYBandglycerol.Asoneofthemostreferencedspermcryopreservationmediaworldwide,FreezingMediumTYBhasestablisheditselfasthestandardforperformance.
•2yearshelflife
•Contains:
-20%eggyolkfromUSDAcertifiedSPF(VirusFree)layingflocks,heatinactivatedat56°Cfor30minutes
-12%glycerol
-10µg/mLgentamicinsulfate
•CEMarked
QualityControlTesting:
•EndotoxinbyLimulusAmebocyteLysate(LAL)
•SpermCryosurvivalassay
•SterilitybythecurrentUSP,CFRandPh.Eur.SterilityTest
•pH
•Osmolality
Storage
StoreBelow-10°C
ShelfLife
2yearsfromdateofmanufacture