SpermMaintenanceMediumusesonly1mLofmediumper3mLofsemenorsperm.Thismoreefficientmediummeanslowercostspercycleofspermcryopreservation,minimaldilutionoflowqualitysemen,andpotentiallyfewerstraws/vialsperpatient.SpermMaintenanceMediumhassimilarperformancetoFreezingMediumTYB,aTEST-yolkbufferbasedspermcryopreservationmedium,withoutcontainingeggyolk(Es-slami,2009).Themedium’suniqueformulationcontainsingredients,suchashypotaurineandascorbicacid,thathavebeenshowntoprotectagainstreactiveoxygenspecies.
•CEMarked
•Contains:
-28%v/vglycerolfora3:1ratioofsementomedium
-Antioxidants,suchashypotaurineandascorbicacid
-HEPES
-HumanSerumAlbumin
-Sucrose
-PluronicF-68
QualityControlTesting:
•EndotoxinbyLimulusAmebocyteLysate(LAL)
•SpermCryosurvivalassay
•SterilitybythecurrentUSP,CFRorPh.Eur.SterilityTest
•pH
•Osmolality
Storage
Storebelow-10°C
ShelfLife
Productshouldbeusedwithin(8)weeksfromopeningwhenstoredundertherecommendedconditionsof2°to8°C.
